Figure 1

Representative 2-DE gels of E. coli BL21 plasmid-bearing strains. Two-dimensional gel electrophoresis (2-DE) of the cell lysates of E. coli BL21 harboring pGEX-2TK-nanA-5R (A, B) and E. coli BL21 harboring pGEX-2TK-2ep-5D (C, D) harvested before (A, C) and after 3-h IPTG induction (B, D). Numbers on the gels reflect the identified protein spots as denoted in Table 1.