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Figure 8 | Microbial Cell Factories

Figure 8

From: Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1

Figure 8

Purification of C-terminally 6xHis-tagged aminotransferase FumI by nickel affinity chromatography. Cell extract of E. coli ArcticExpress(DE3) [pET-30a-AT144HIS] after production of FumI-HIS (48.3 kDa) was subjected to nickel affinity chromatography using 20 mM (A), 60 mM (B) or 100 mM (C) imidazole in binding buffer. SDS-PAGE analysis (12% polyacrylamide, Coomassie Brilliant Blue stained) of samples corresponding to these chromatograms show the clarified cell lysate that was applied to the column (CL), samples of the 1-ml fractions that eluted after the indicated volume, and a molecular mass marker (M).

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