Figure 5From: Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1Purification of MBP-FumI fusion protein by affinity chromatography. Chromatogram (A) of affinity chromatography of MBP-FumI fusion protein (89.5 kDa) on dextrin sepharose and elution with 50 mM maltose, and analysis of fractions by SDS-PAGE (10% polyacrylamide, GelCode Blue staining) (B). Lane CL, clarified cell lysate; lane 2, fraction from 2 to 2.5 ml; lane 23, fraction from 23 to 23.5 ml and so forth; lane M, molecular mass marker.Back to article page