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Figure 4 | Microbial Cell Factories

Figure 4

From: Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1

Figure 4

Solubility of MBP-FumI fusion protein produced in E. coli (DE3) at 30°C or 23.5°C. SDS-PAGE (10% polyacrylamide, silver staining) analysis of total (T), soluble (S) and insoluble (I) fractions of cell lysates of E. coli Origami 2(DE3) after expression of MBP-FumI fusion protein (89.5 kDa) from vector pMAL-AT144, or MBP (50.9 kDa) from vector pMAL (pMAL-c2E, no insert). Expression was done for 20 h at room temperature (RT, 23.5°C) (A) or 30°C (B). Lane M, molecular mass marker.

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Microbial Cell Factories

ISSN: 1475-2859

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