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Figure 3 | Microbial Cell Factories

Figure 3

From: Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1

Figure 3

Comparison of E. coli (DE3) host strains and effect of IPTG concentrations on FumI production at 30°C. Western blot (anti-His6-antibody; from 12% polyacrylamide SDS-PAGE gels) analysis of N-terminally 6xHis-tagged aminotransferase FumI. The fumI gene was expressed in E. coli BL21(DE3) (A), E. coli HMS174(DE3) (A), E. coli Rosetta 2(DE3) (B), E. coli Rosetta-gami(DE3) (B) and E. coli Tuner(DE3) (C) from vector pET-3a-HISAT at 30°C without (-) and with addition of IPTG (10 μM, 50 μM, 100 μM, 400 μM). Lane S, soluble fraction of cell lysate; Lane I, insoluble fraction; Lane M, molecular mass marker. The arrow indicates the band corresponding to the molecular mass of His-tagged aminotransferase FumI (47.5 kDa).

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