Figure 3From: Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1Comparison of E. coli (DE3) host strains and effect of IPTG concentrations on FumI production at 30°C. Western blot (anti-His6-antibody; from 12% polyacrylamide SDS-PAGE gels) analysis of N-terminally 6xHis-tagged aminotransferase FumI. The fumI gene was expressed in E. coli BL21(DE3) (A), E. coli HMS174(DE3) (A), E. coli Rosetta 2(DE3) (B), E. coli Rosetta-gami(DE3) (B) and E. coli Tuner(DE3) (C) from vector pET-3a-HISAT at 30°C without (-) and with addition of IPTG (10 μM, 50 μM, 100 μM, 400 μM). Lane S, soluble fraction of cell lysate; Lane I, insoluble fraction; Lane M, molecular mass marker. The arrow indicates the band corresponding to the molecular mass of His-tagged aminotransferase FumI (47.5 kDa).Back to article page