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Figure 2 | Microbial Cell Factories

Figure 2

From: Enhancement of solubility in Escherichia coli and purification of an aminotransferase from Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1

Figure 2

Expression of fumI in E. coli BL21(DE3) at 37°C. SDS-PAGE (12% polyacrylamide, GelCode Blue staining) analysis of fractions of cell lysate of E. coli BL21(DE3) expressing fumI from vector pET-30a-AT144 by induction with 1 mM IPTG at 37°C. Lane M, molecular mass marker; Lane T, total cell lysate; Lane S, soluble fraction of cell lysate; Lane I, insoluble fraction. The molecular mass of aminotransferase FumI (46.3 kDa) is indicated.

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