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Figure 6 | Microbial Cell Factories

Figure 6

From: Production of glycoprotein vaccines in Escherichia coli

Figure 6

Production of EPA-O1 in fed-batch culture. Cultivation and induction were performed according to strategy C as described in Materials and methods and legend to Figure 5. Strain: E. coli CLM24 (pGVXN150, pGVXN64, pGVXN114). (A) Logarithmic growth curve (circles) and time course of biomass concentrations (squares). Filled symbols, dotted line: fed-batch run 1 (FB1); open symbols, solid line: fed-batch run 2 (FB2). Time 0 h (broken, vertical line): induction with L-arabinose and IPTG. (B) Time course of EPA and EPA-O1 formation in fed-batch culture compared to LB shake flask culture (SF); anti-EPA Western blots, normalized samples, numbers indicate time after induction.

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