Figure 4
From: Production of glycoprotein vaccines in Escherichia coli
Chemostat cultivation ( D = 0.1 h-1) of AcrA-O1 producing E. coli using different growth substrates. (A) Time course of optical density after inoculation, bars indicate the period of L-arabinose (ara) co-feed and arrows indicate the time point of the addition of 1 mM IPTG. (B) Glycoconjugate formation in chemostat cultures analysed with anti-AcrA antibodies on Western blot (normalized samples). (C) Expression of pglB in LB chemostat culture compared to batch culture (anti-HA Western blot, normalized samples).