Figure 3
From: Production of glycoprotein vaccines in Escherichia coli
Growth and glycoconjugate formation in batch culture. AcrA-O1 producing E. coli CLM24 (pMIK44, pGVXN64, pGVXN114) were cultivated in LB medium in a 2 L-bioreactor. The arrow indicates the time point of induction with 2 g L-1 L-arabinose and 1 mM IPTG. Normalized total cell protein samples were taken at the indicated time points (a to f) and analysed by Western blot using anti-ArcA antibodies.