Figure 2
From: Production of glycoprotein vaccines in Escherichia coli
Glycosylation of AcrA and EPA with Shigella O1 polysaccharides. Extracts of periplasmic proteins from E. coli CLM24 expressing carrier protein, Shigella polysaccharides (pGVXN64) and either wild-type (wt; pGVXN114) or inactive PglB (mut; pGVXN115) were analysed by Western blot. Lanes 1 and 2: AcrA-expressing strain (pMIK44) analysed with anti-AcrA antibodies; lanes 3 and 4: AcrA-expressing strain analysed with anti-Shigella O1 antibodies (same SDS-polyacrylamide gel as lanes 1 and 2); lanes 5 and 6: EPA-expressing strain (pGVXN150) analysed with anti-EPA antibodies; lanes 7 and 8: EPA-expressing strain analysed with anti-Shigella O1 antibodies (same SDS-polyacrylamide gel as lanes 5 and 6).