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Figure 5 | Microbial Cell Factories

Figure 5

From: Automated assay for screening the enzymatic release of reducing sugars from micronized biomass

Figure 5

Calibration of the sugar releasing experiment using micronized wheat-straw (left) or spruce (right). Except in D, black-square refers to raw data. Continuous line (A, B), open circle or cross (C) refer to background. White-square refers to raw data minus background. Standard deviations of triplicate experiments are indicated. Background curves were made of single measures. For each parameter, the value considered optimal and selected for subsequent experiments is flagged on the X axis by a vertical dotted line. A, Amount of reducing sugars released from micronized biomass as a function of substrate concentration. OD540 provided by the enzymatic digestion of 1% (wheat-straw) or 2% (spruce) substrate suspension are indicated on raw data curves. B, Amount of reducing sugars released from micronized biomass as a function of enzyme (E508) concentration. The continuous line is the "enzyme background". C, amount of reducing sugars released from micronized biomass as a function of incubation time. "Substrate background" (open circle), and "enzyme background" (cross) lines are reported. D, As for C except that only net sugar release curves are shown. Incubations were performed at 37°C (white square) or 50°C (black square). Inset: μmol of glucose equivalent are the values obtained at 24 h and 72 h incubation for wheat-straw and spruce, respectively (values in brackets are standard deviations); initial rates were calculated using data from 0 to 4 h time points, and were expressed as μmol glucose equivalent per hour (values in brackets are correlation factors).

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