Figure 2

Protein sequence alignment of pyranose oxidase from Lyophyllum shimeji , Ls P2Ox [GenBank: BAD12079], Phanerochaete chrysosporium , Pc P2Ox [GenBank: AAS93628] and Trametes multicolor, Tm P2Ox [GenBank: AAX09279.1]. The active-site loop, which is important for substrate recognition and catalysis, is marked by a dark-blue background, while residues that are proposed to be involved in substrate binding and/or catalysis are highlighted in red. Residues that are strictly conserved in these three sequences are shaded black, while conserved residues found in two of these sequences are highlighted in grey.