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Figure 3 | Microbial Cell Factories

Figure 3

From: Development of a Bacillus subtilis expression system using the improved Pglv promoter

Figure 3

Reconstruction of B. subtilis host. (A) Agarose-gel-electrophoresis of chromosome DNA digested with Apa I and Eco RI. Lane1, treated chromosome DNA from B. subtilis 1A747 [this served as the negative control]; lanes 2 and 3, treated chromosome DNA from B. subtilis BCYL; lane 4, molecular weigh marker. (B) Southern blot analysis of recombinant B. subtilis BCYL chromosome DNA. The lanes 1, 2 and 3 are that the same as in (A). (C) β-Gal production by the improved expression system. White triangle and white square represents the β-Gal production by B. subtilis BCYL harboring pJRINM1 cultures in LB with 5% maltose and LB with 5% maltose plus 5% glucose, respectively; black triangle and black square represents the β-Gal production by B. subtilis 1A747 harboring pJRINM1 when cultured in LB with 5% maltose and LB with 5% maltose plus 5% glucose, respectively.

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