Figure 3
From: Production of biohydrogen by recombinant expression of [NiFe]-hydrogenase 1 in Escherichia coli
(A) In vivo H 2 production according to metal content and (B) in vitro hydrogenase 1 activity according to metal content and cellular fraction. Culture was performed in 500 mL serum bottles containing 400 mL M9CA medium. The metal composition was set as four combinations, specifically, (Ni 0 μM, Fe 0 μM), (Ni 30 μM, Fe 0 μM), (Ni 0 μM, Fe 30 μM), (Ni 30 μM, Fe 30 μM). Cell fractionation was performed as described in Materials and methods. Other conditions were similar to those described for Fig. 2. Each value and error bar represents the mean of triplicate samples and standard deviation.