Figure 4From: The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteinsRT-PCR on UPR induced and non-induced cultures. The HAC1 mRNA is constitutively spliced. Also when the temperature is decreased from 30°C to 20°C we do not detect unspliced HAC1 mRNA (+ and - indicate growth in the presence or absence of 5 mM DTT respectively). Lane 6 is the result from a PCR performed on gDNA showing that the intron is clearly present in the genomic DNA sequence. The ladder on the gel is the 1 kb DNA marker from Promega.Back to article page