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Table 4 Effect of shaking rate and OD600 at the first induction on C-P4H production

From: Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase

 

C-P4H (ng μg-1 total protein)

 

5.5

12.5

24.5

Shaking rate

-

-

-

OD600 at the fist induction

-

(-)

(-)

interaction

yes

yes

yes

 

C-P4H (mg L-1)

 

5.5

12.5

24.5

Shaking rate

(-)

(-)

(-)

OD600 at the fist induction

(-)

+

(+)

interaction

yes

yes

(yes)

  1. - negative effect of high level parameter
  2. + positive effect of high level parameter
  3. () effect is not significant, tendency is shown in parentheses
  4. yes interaction exists