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Table 4 Effect of shaking rate and OD600 at the first induction on C-P4H production

From: Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase

  C-P4H (ng μg-1 total protein)
  5.5 12.5 24.5
Shaking rate - - -
OD600 at the fist induction - (-) (-)
interaction yes yes yes
  C-P4H (mg L-1)
  5.5 12.5 24.5
Shaking rate (-) (-) (-)
OD600 at the fist induction (-) + (+)
interaction yes yes (yes)
  1. - negative effect of high level parameter
  2. + positive effect of high level parameter
  3. () effect is not significant, tendency is shown in parentheses
  4. yes interaction exists