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Table 3 Optimization of C-P4H production: summary of cultivation conditions

From: Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase

shaking rate [rpm] 1st induction at OD600 =
220 (high level) 3 (low level)
220 (high level) 10 (high level)
220, 100 after 1st induction (low level) 3 (low level)
220, 100 after 1st induction (low level) 10 (high level)