Table 3 Optimization of C-P4H production: summary of cultivation conditions
From: Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase
shaking rate [rpm] | 1st induction at OD600 = |
|---|---|
220 (high level) | 3 (low level) |
220 (high level) | 10 (high level) |
220, 100 after 1st induction (low level) | 3 (low level) |
220, 100 after 1st induction (low level) | 10 (high level) |