Table 2 Optimal antibody concentrations
From: Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase
Amount of C-P4H, ng | FLU signal/background | Dilutions of antibody | ||
|---|---|---|---|---|
mab-α | pab-β | GAR-AP | ||
1 | 13.37 | 1/250 | 1/770 | 1/2500 |
20 | 75.78 | 1/250 | 1/710 | 1/2500 |
50 | 88.77 | 1/250 | 1/830 | 1/3300 |