Figure 4From: Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylaseOptimization of hybridization and washing procedures of the C-P4H sandwich ELISA. (A) Optimization of the hybridization time. (B) Optimization of the washing conditions (shaking rate and NaCl concentration in the washing buffer). 1 ng of purified C-P4H was used as target. The error bars show the ± SD of three parallel experiments.Back to article page