Figure 2
From: Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase
Attachment of mab-α to magnetic beads and influence of mab-α and GAM-AP dilutions on fluorescent signal. (A) The principle of the test. The mab-α binds to magnetic beads via biotin-streptavidin interaction. The complex is detected with the Goat Anti-Mouse IgG labeled with AP (GAM-AP). (B) The generated signals obtained when different mab-α and GAM-AP concentrations were used. The error bars show the ± SD of three parallel experiments.