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Table 1 Fps1 can be produced in a range of microbial cell factories

From: Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory

Production Host

Promoter

Production

Localisation

Reference

S. cerevisiae W301-1A

GAL1

Yes

tm

[38]

S. cerevisiae W301-1A

TPI1

Yes

tm

[38]

S. cerevisiae 954 VW K70

TPI1

Yes

tm

[21] and this study

S. cerevisiae KOY.PK2-1C82

TPI1

Yes

tm

This study

S. cerevisiae TM6*

TPI1

Yes

tm

This study

P. pastoris X-33

AOX1

Yes

pm

[38]

P. pastoris X-33

AOX1

Yes†

nd

[38]

P. pastoris GS115

AOX1

Yes

nd

[38]

P. pastoris KM71

AOX1

Yes

nd

[38]

E. coli JM109

lac

Yes

tm

[38]

E. coli JM109

T7/lac

Yes

tm

[38]

  1. Fps1 was produced in S. cerevisiae, P. pastoris and E. coli as previously described [21, 38] and as a part of this study (tm, total membrane; pm, plasma membrane; nd, not determined). Note that † indicates that the α-factor signal peptide was used in the expression construct. In all cases prior to this study, total yields were less than 50 μg/L, which was below the threshold value for further study [21, 38].