MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms

Table 2 Comparison of obtained results covering three domains of living organisms

	Eukarya		Bacteria		Archaea		Total
	pFO4	pGEX	pFO4	pGEX	pFO4	pGEX	pFO4	pGEX	combined^c
number of soluble proteins	14	13	13	25	18	26	45	64	84
number of insoluble proteins	10	19	4	5	12	24	26	48	34
no expression^a	42	37	11	2	31	11	84	50	52
no clone^b	8	5	4	0	9	12	37	30	22
percentage of soluble proteins	7%	7%	7%	13%	9%	14%	23%	33%	44%
percentage of soluble + insoluble proteins	13,00%	17%	9%	16%	16%	26%	37%	58%	61%

^a number of targets for which no expected bands were visualized on SDS-PAGE gels
^b number of target genes did not clone in expression vectors
^c number of different soluble proteins obtained in pGEX or in pFO4 vectors

ISSN: 1475-2859