Figure 3
Up-scale and purification of targets from P. abyssi. a. SDS-PAGE gel of up-scaled and purified HIS-tagged targets F75, F124, F127 and F167 before and after final polishing on Co2+ specific beads (SN = Supernatant of cell culture; B = after incubation with Dynabeads TALON; M = protein markers; F75 = test line; NI = F75 without induction). b. 12% SDS-PAGE analysis of a P. abyssi RadA (PAB0164, target 92 round R1) pull-down experiment. Proteins identified using an Ultraflex MALDI-TOF/TOF instrument (Bruker Daltonics) are indicated with arrows.