Strain/Plasmid | Genotype/Description | Source/Reference |
---|---|---|
Bacterial strains | ||
Planobispora rosea ATCC53773 | Biosearch Italia SpA, Gerenzano | |
Streptomyces lividans ZX7 | pro-2 str-6 rec-46 Δdnd HAU3 SLP2- SLP3- | John Innes Centre, Norwich, UK |
Streptomyces laurentii | German Collection of Microorganisms and Cell Cultures, DSMZ GmbH, Braunschweig, Germany | |
Streptomyces coelicolor M145 | John Innes Centre, Norwich, UK | |
Escherichia coli DH10B | F- mcrA Δ(mrr- hsdRMS-mcr BC) Δ80dlacZDM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu) 7697 galU GalK D- rpsL nupG | Life Technologies, Gibco BRL. |
EC-40 | E. coli containing ESAC40 | Alduina et al., 2003 |
SC-3.5 | S. coelicolor containing ESAC3.5 | This study |
SL-ESAC | S. lividans containing ESAC | Alduina et al., 2003 |
SL-40 | S. lividans containing ESAC40 | Alduina et al., 2003 |
SL-3.5 | S. lividans containing ESAC3.5 | This study |
SL-Abc | S. lividans containing ESACAbc | This study |
SL-Imp | S. lividans containing ESACImp | This study |
SL-Abc/Imp | S. lividans containing ESACAbc and pNImp | This study |
SL-Imp/Abc | S. lividans containing ESACImp and pNAbc | This study |
Plasmids | ||
pUC | pUC18 | |
pBS | pBluescript | |
ESAC | Streptomyces-E. coli Artificial Chromosome | Sosio et al., 2002 |
ESAC40 | ESAC containing a P.rosea 40 kb fragment | Alduina et al., 2003 |
ESAC3.5 | ESAC containing a P.rosea 3.5 kb fragment with abc and imp genes | This study (fig.1) |
ESACAbc | ESAC3.5/imp::pBS | This study (fig.1) |
ESACImp | ESAC3.5/abc::pUC | This study (fig.1) |
pN702GEM3 | Fernández-Abalos et al., 2003 | |
pNAbc | pN702 containing abc | This study |
pNImp | pN702 containing imp | This study |