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Table 1 Strains and plasmids.

From: Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracellular medium

Strain/Plasmid

Genotype/Description

Source/Reference

Bacterial strains

  

Planobispora rosea ATCC53773

 

Biosearch Italia SpA, Gerenzano

Streptomyces lividans ZX7

pro-2 str-6 rec-46 Δdnd HAU3 SLP2- SLP3-

John Innes Centre, Norwich, UK

Streptomyces laurentii

 

German Collection of Microorganisms and Cell Cultures, DSMZ GmbH, Braunschweig, Germany

Streptomyces coelicolor M145

 

John Innes Centre, Norwich, UK

Escherichia coli DH10B

F- mcrA Δ(mrr- hsdRMS-mcr BC) Δ80dlacZDM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu) 7697 galU GalK D- rpsL nupG

Life Technologies, Gibco BRL.

EC-40

E. coli containing ESAC40

Alduina et al., 2003

SC-3.5

S. coelicolor containing ESAC3.5

This study

SL-ESAC

S. lividans containing ESAC

Alduina et al., 2003

SL-40

S. lividans containing ESAC40

Alduina et al., 2003

SL-3.5

S. lividans containing ESAC3.5

This study

SL-Abc

S. lividans containing ESACAbc

This study

SL-Imp

S. lividans containing ESACImp

This study

SL-Abc/Imp

S. lividans containing ESACAbc and pNImp

This study

SL-Imp/Abc

S. lividans containing ESACImp and pNAbc

This study

Plasmids

  

pUC

pUC18

 

pBS

pBluescript

 

ESAC

Streptomyces-E. coli Artificial Chromosome

Sosio et al., 2002

ESAC40

ESAC containing a P.rosea 40 kb fragment

Alduina et al., 2003

ESAC3.5

ESAC containing a P.rosea 3.5 kb fragment with abc and imp genes

This study (fig.1)

ESACAbc

ESAC3.5/imp::pBS

This study (fig.1)

ESACImp

ESAC3.5/abc::pUC

This study (fig.1)

pN702GEM3

 

Fernández-Abalos et al., 2003

pNAbc

pN702 containing abc

This study

pNImp

pN702 containing imp

This study