Table 1 Steady-state kinetics for C. magnoliae JH110 ER
Substrate | Specific activity (U/mg protein) | Km(mM) | kcat(S-1) | kcat/Km(mM-1S-1) |
|---|---|---|---|---|
p-Nitrobenzaldehyde | 4.3 ± 0.3 | 0.17 ± 0.01 | 1.4 ± 0.1 | 8.2 |
D-Erythrose | 16.5 ± 1.3 | 8.5 ± 0.4 | 7.6 ± 0.4 | 0.89 |
D-Xylose | 0.96 ± 0.06 | 72 ± 3.4 | 1.8 ± 0.1 | 0.011 |
D-Ribose | 0.66 ± 0.01 | 56 ± 4.1 | 1.7 ± 0.2 | 0.030 |
D-Glucose | N.D. | N.D. | N.D. | N.D. |
D-Fructose | N.D. | N.D. | N.D. | N.D. |
D-Galactose | N.D. | N.D. | N.D. | N.D. |
NADH | N.D. | N.D. | N.D. | N.D. |
NADP+ | 6.4 ± 0.5 | 0.24 ± 0.04 | 0.84 ± 0.06 | 3.5 |
NADPH | 81.3 ± 2.7 | 0.016 ± 0.01 | 48 ± 2.5 | 3000 |