Figure 4

Determination of pH and temperature optima. (A) Effect of temperature on the specific activity of the CmER. For the effect of temperature, the activity of CmER was determined at different temperatures between 15 and 55°C at pH 5.5. (B) pH dependence of CmER activity. The effect of pH on the activity of CmER was determined by assaying the enzyme activity at various pH values (pH 4.0 - 9.0). The reactions were carried out at 37°C in the following buffer systems and pH values: sodium citrate buffer (pH 4.5 - 6.0), potassium phosphate buffer (pH 6.0 - 8.0), Tris-HCl (pH 8.0 - 9.0), and glycine-NaOH buffer (pH 9.0 - 10.0). The assays were performed in triplicate at each temperature and pH point.