Figure 5

Cultivation of E. coli RB791 pAdh expressing a heterologous alcohol dehydrogenase in the CultiBag RM system with 1 L of EnBase^® Flo medium with the addition of complex additives (EnBase^® Booster). (A) In this cultivation the amylase concentration was stepwise increased from 1.5 to 6 U L^-1; the boosting solution was added three times. Protein expression was induced with 1 mM IPTG after 27 hours. (B) Coomassie Brilliant Blue stained 12% SDS polyacrylamide gel of soluble and insoluble protein samples. Same amounts of cells were applied to each lane. Lane 1) before induction; 2) at induction; 3) 11 h; 4) 16 h; 5) 21 h after induction.