Figure 4
Cultivation of E. coli RB791 pAdh in EnBase® Flo with the CultiBag RM system. (A) Data of a cultivation in a 2 L bag with 1 L EnBase® Flo medium containing 1.5 U L-1 of amylase. Trace elements and MgSO4 were added after 18 and 41 hours and ammonium sulfate was added after 26 hours. Protein expression was induced by 1 mM IPTG after 26 hours. (B) Coomassie Brilliant Blue stained SDS polyacrylamide gel with soluble (left lanes) and insoluble protein samples (right lanes). Same amounts of cells were loaded onto each lane. Lane 1) before induction; 2) at induction; 3) after 12 h; 4) after 17 h; 5) after 20 h. Most left lane - size standard.