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Figure 2 | Microbial Cell Factories

Figure 2

From: High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor

Figure 2

DO-stat based Fed-batch cultivation of E. coli BL21 in minimal medium with the CultiBag RM system. Cultivation conditions: 10 L bag with 4 L filling volume, maximum rocking angle, 35 rocks min-1 during the batch phase and the maximum rocking speed (42 rocks min-1) during the feeding phase, cultivation temperature 25°C during the batch phase and increase to 37°C after 11 hours. The pO2 was controlled to 50% by a dual control of (i) the gas flow rate from 1 to 6 L min-1 and (ii) pulsing of pure oxygen into the inlet air stream (up to 100%, maximum total flow rate of 6 L min-1, named O2 ratio).

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