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Figure 1 | Microbial Cell Factories

Figure 1

From: High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor

Figure 1

Batch cultivation of E. coli BL21 in glucose mineral salt medium in (A) a shake flask with the Sensolux monitoring system and (B, C) with the CultiBag RM system. Cultivation conditions: 10 L bag with 5 L working volume, maximum rocking angle, 35 rocks per min, 37°C, air flow rate 1 L min-1 in (B). In (C) the air flow rate was 1 L min-1 at the start of the cultivation. After the pO2 decreased to 50% a dual controller mode was started to maintain the pO2 by (i) increasing the air flow rate to 6 L min-1 and (ii) pulse addition of pure oxygen into the inlet air stream (up to 100%).

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