Figure 8
From: Quality control of inclusion bodies in Escherichia coli
Northern Blot analyses (A-C) of the α-glucosidase transcript with different oligonucleotide probes. Analyses were performed with samples from E. coli RB791 pKK177glucC pUBS520 collected from batch cultivations 60 min after induction. Different probes were tested, covering the glucC mRNA sequence from position 374 -to 399 (A), 1160 to 1185 (B), and from position 1580 to 1605 (C). The right side of the figure (D) indicates schematically the different locations of the analysed mRNA fragments in relation to the glucC sequence. The probe locations are indicated by red bars. Non-translated 5' regions of the mRNA transcript are shown as a dashed line.