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Table 1 Plasmids and S. cerevisiae strains used in this study

From: PGM2 overexpression improves anaerobic galactose fermentation in Saccharomyces cerevisiae

 

Relevant genotype/phenotype

Reference

Plasmids

  

YEplacHXT

YEplac195, HXT7p-PGKt URA3

[27]

YEplacHXT-PGM2

YEplac195, HXT7p-PGM2-PGKt URA3

This work

YDp-H

HIS3

[33]

YIplac211

URA3

[30]

YIplac211 HXT-PGM2

Ylpac211, HXT7p-PGM2-PGKt URA3

This work

S. cerevisiae strains (*)

  

CEN.PK 113-11C

MAT a his3Δ1 ura 3-52 MAL2-8c SUC2

[28]

TMB 3126

CEN.PK 113-11C, his3Δ1 YEplacHXT URA3

This work

TMB 3400

genomic S. cerevisiae DNA (used as template to amplify PGM2)

[29]

TMB 3127

CEN.PK 113-11C, his3Δ1 YEplacHXT-PGM2 URA3

This work

TMB 3128 "Control m"

TMB 3126 his3::HIS3 YEplacHXT URA3

This work

TMB 3129 "PGM2 m"

TMB 3127 his3::HIS3 YEplacHXT-PGM2 URA3

This work

TMB 3134

CEN.PK 113-11C, his3::HIS3 ura3

This work

TMB 3135 "Control i"

CEN.PK 113-11C, his3::HIS3 ura3::URA3

This work

TMB 3136 "PGM2 i"

CEN.PK 113-11C, his3::HIS3 ura3::URA3 HXT7'p-PGM2-PGKt

This work

  1. (*) Abbreviations of the most important genetic features are shown in quotation marks