From: Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus
Primer ID | Sequence | Details |
---|---|---|
PSPGOXF | 5' - TTTTTGCTGTCATTCGTTCAAGGTAAAAGA AGCAATGGCATTGAAGCC | Underlined sequence is homologous to the last bases of K. lactis killer protein k1 secretion signal sequence |
PSPGOXR | 5' - CACCACCACCAGTAGAGACATGGGAGATCG TCACTGCATGGAAGCATA | Underlined sequence is homologous to the first bases of S. cerevisiae PGK terminator |
PINU1Fb | 5'- GGATCC GAATTCTCAAACCGAAATGGG | Underlined extremity is an added Bam HI restriction site |
PINU1Rb | 5'- GGATCC ACGCCAGACGTCTTGTGTCCG | Underlined extremity is an added Bam HI restriction site |
PINUGOXF | 5' -GTCAGTGCTTCAGTTATCAATTACAAGAGA AGCAATGGCATTGAAGCC | Underlined sequence is homologous to the last bases of K. marxianus INU1 secretion signal sequence |
PINUGOXR | 5'- TTTTGTCGTTAGTAAAGTAAGCAGATCAGA TCACTGCATGGAAGCATA | Underlined sequence is homologous to the last bases of K. marxianus INU1 terminator |