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Table 5 Primers.

From: Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus

Primer ID

Sequence

Details

PSPGOXF

5' - TTTTTGCTGTCATTCGTTCAAGGTAAAAGA AGCAATGGCATTGAAGCC

Underlined sequence is homologous to the last bases of K. lactis killer protein k1 secretion signal sequence

PSPGOXR

5' - CACCACCACCAGTAGAGACATGGGAGATCG TCACTGCATGGAAGCATA

Underlined sequence is homologous to the first bases of S. cerevisiae PGK terminator

PINU1Fb

5'- GGATCC GAATTCTCAAACCGAAATGGG

Underlined extremity is an added Bam HI restriction site

PINU1Rb

5'- GGATCC ACGCCAGACGTCTTGTGTCCG

Underlined extremity is an added Bam HI restriction site

PINUGOXF

5' -GTCAGTGCTTCAGTTATCAATTACAAGAGA AGCAATGGCATTGAAGCC

Underlined sequence is homologous to the last bases of K. marxianus INU1 secretion signal sequence

PINUGOXR

5'- TTTTGTCGTTAGTAAAGTAAGCAGATCAGA TCACTGCATGGAAGCATA

Underlined sequence is homologous to the last bases of K. marxianus INU1 terminator

  1. List of primers used on DNA constructions.