Figure 2
From: Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus
Coomassie blue stained SDS-PAGE for the analysis of the glycosylation pattern of GOX expressed in (A, B) K. marxianus and (C, D) K. lactis. (A) K. marxianus cell wall-associated GOX; (B) K. marxianus supernatant GOX; (C) K. lactis cell wall-associated GOX; (D) K. lactis supernatant GOX. Glyc: glycosylated enzyme. Deglyc: deglycosylated enzyme with PNGase F, as described in Methods. Construction codes are listed in Table 1. Standard: commercial GOX from A. niger (Product B6916, Sigma, USA), solubilized in water (Glyc) or deglycosylated (Deglyc) as described above. The arrows indicate the two bands resulting from the deglycosylation of GOX expressed in K. marxianus.