Figure 1
From: Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus
Expression systems constructed in this work. (A) pSPGOX provides episomal expression of GOX under control of the ScPGK promoter; (B) pSPINGOX is an episomal vector and GOX expression is performed under control of the KmINU1 promoter; (C) pINGOXi is an integrative plasmid that confers the cells capacity of GOX expression under control of the KmINU1 promoter. (A) and (B) derive from pSPGK1 [48] and (C) corresponds to pNADFL11 [20] plus the expression cassette. Legend: p, promoter; t, terminator; SS, secretion signal sequence.