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Table 1 Effects of metal ions and chemicals on the purified recombinant AiiAB546 activity

From: High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture

Chemicals

Relativity activity (%)a

Chemicals

Relativity activity (%)a

 

1 mM

10 mM

 

1 mM

10 mM

None

100

100

Mg2+

64.8 ± 5.2*

108.4 ± 2.5*

Na+

109.5 ± 5.2*

104.6 ± 2.4*

Fe3+

104.8 ± 2.6*

103.3 ± 1.7*

K+

104.5 ± 2.9*

109.3 ± 6.1*

Zn2+

89.3 ± 4.2*

109.5 ± 5.9*

Ca2+

106.5 ± 3.7*

117.5 ± 1.2*

Mn2+

107.6 ± 4.9*

108.4 ± 5.8*

Li+

115.4 ± 3.9*

65.8 ± 5.4*

Ni2+

103.4 ± 4.4

102.6 ± 3.5

Co2+

118.5 ± 7.1*

103.8 ± 4.3

Pb2+

108.6 ± 3.9*

87.5 ± 3.6*

Cu2+

68.5 ± 7.2*

58.8 ± 5.4*

EDTA

66.5 ± 2.5*

103.6 ± 3.3*

Cr3+

29.1 ± 4.2*

49.6 ± 3.8*

SDS

73.2 ± 4.2*

0.0 ± 5.2*

Ag+

0.0 ± 5.2*

0.0 ± 3.7*

β-Mercaptoethanol

112.2 ± 3.8

92.0 ± 5.1*

Hg2+

0.0 ± 3.9*

0.0 ± 4.9*

   
  1. a Values represent the means ± SD (n = 3) relative to the untreated control samples, and those marked with "*" are significantly different (one-way ANOVA; P < 0.05) from that of control without addition of chemicals.