Figure 3
Enzyme characterization of purified recombinant AiiA B546 . a: Effect of pH on the AHL-lactonase activity of AiiAB546. b: pH stability assay. After pre-incubating the enzyme at 25°C for 1 h in buffers of pH 5.0-12.0, the residual activity was measured in PBS buffer (pH 8.0) at 25°C. c: Effect of temperature on AHL-lactonase activity. d: Thermostability of recombinant AiiAB546. The enzyme was pre-incubated at 60 and 70°C in PBS buffer (pH 8.0), and aliquots were removed at specific time points for the measurement of residual activity at 25°C.