Table 1 Plasmids used in this study.

pLB145

Cm^R, pWV01 derivative carrying exp4_SP-nucB gene fusion under the control of ZitR-regulated P_Zn promoter

[13]

pGTPb_102a

Amp^R, pFastBacHta (Invitrogen Paisley UK) derivative carrying an ORF for HFFT tag (His Flag FoldFold Flag Tev) [56–58]

This study

pGTPb_SP_Exp4

Amp^R, pGTPb_102a derivative where the ORF for Exp4 signal peptide (SP_Exp4) has been cloned

This study

pGTPb_P_Zn zitR- SP_Exp4

Amp^R, pGTPb_SP_Exp4 derivative where zitR is in operon with the ORF for SP_Exp4 under P_Zn control

This study

pGTP_FZ301

Cm^R, pLB145 derivative where a multi-cloning site has been cloned after the ORF for SP_Exp4 (in operon with zitR under P_Zn control)

This study

pGTP_FZ301_NucB

Cm^R, pGTP_FZ301 derivative where nucB ORF has been cloned leading to a gene fusion encoding SP_Exp4-NucB precursor and expressed under the control of ZitR-regulated P_Zn promoter

This study