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Figure 1 | Microbial Cell Factories

Figure 1

From: Production and purification of staphylococcal nuclease in Lactococcus lactis using a new expression-secretion system and a pH-regulated mini-reactor

Figure 1

Expression-secretion vector used for rNuc production. (A) pGTP_FZ_301 allows any open reading frame to be cloned as a translational fusion to Exp4 signal peptide coding sequence, and expressed as an operon to zitR under the control of PZn promoter. (B) pGTP_FZ_301_NucB results from the cloning of a recombinant truncated form of S. aureus nuc ORF (coding for NucB) into pGTP_FZ_301. NucB, the secreted mature form of staphylococcal Nuc (after signal peptide cleavage) is fused in frame to Exp4 signal peptide, leading to a recombinant precursor that is secreted as rNuc. MCS: Multi-Cloning Site; RBS: Ribosome Binding Site; term: terminator

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