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Table 2 Primers used for amplification the GPD2 disruption cassette and verification of its correct genomic integration

From: Quantitative evaluation of yeast's requirement for glycerol formation in very high ethanol performance fed-batch process

No. Function Sequence
P60* Forward primer for amplification of GPD2 deletion cassette 5'-TAGCTTACGGACCTATTGCCATTGTATTCCGATTA ATCTATTGTcagctgaagcttcgtacgc-3'
P61* Reverse primer for amplification of GPD2 deletion cassette 5'-CACATTCTCACCTCTGGCTCGAAGATATGGGAATGCAATTCTGTgcataggccactagtggatctg-3'
P62 Forward primer for verification of GPD2 deletion 5'-ACGATGG CTCTGCCATT-3'
P63 Reverse primer for verification of GPD2 deletion 5'-GATCAGGATCGGCCACTA-3'
  1. *Sequences in low chase letters correspond to the primer sequences for the amplification of the loxP-bleR-loxP deletion cassette from the plasmid pUG66 used as a template [32]. Nucleotides in capital letters are derived from S. cerevisiae S288c genome sequence for integration of the deletion cassette by homologous recombination at the GPD2 locus.