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Table 2 Primers used for amplification the GPD2 disruption cassette and verification of its correct genomic integration

From: Quantitative evaluation of yeast's requirement for glycerol formation in very high ethanol performance fed-batch process

No.

Function

Sequence

P60*

Forward primer for amplification of GPD2 deletion cassette

5'-TAGCTTACGGACCTATTGCCATTGTATTCCGATTA ATCTATTGTcagctgaagcttcgtacgc-3'

P61*

Reverse primer for amplification of GPD2 deletion cassette

5'-CACATTCTCACCTCTGGCTCGAAGATATGGGAATGCAATTCTGTgcataggccactagtggatctg-3'

P62

Forward primer for verification of GPD2 deletion

5'-ACGATGG CTCTGCCATT-3'

P63

Reverse primer for verification of GPD2 deletion

5'-GATCAGGATCGGCCACTA-3'

  1. *Sequences in low chase letters correspond to the primer sequences for the amplification of the loxP-bleR-loxP deletion cassette from the plasmid pUG66 used as a template [32]. Nucleotides in capital letters are derived from S. cerevisiae S288c genome sequence for integration of the deletion cassette by homologous recombination at the GPD2 locus.