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Figure 7 | Microbial Cell Factories

Figure 7

From: Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures

Figure 7

SDS - PAGE images of different cellular protein fractions after EnBase® fed-batch cultivation of E. coli RV308/p ibpfxsT7lucA /pCTUT7MBP-RI in shake flasks at 37°C with induction by 0.5 mM IPTG induced at different specific growth rates (A-C) and during batch production with MSM, semi synthetic medium or LB (D). Lane abbreviations: (T) - total, (S) - soluble (IN) insoluble protein fractions. Numbered SDS-PAGE gel lanes represent: 1 - total protein fraction 10 min before induction, 2 - soluble protein fraction 4 h after induction, 3 - total protein fraction 4 h after induction. Lanes 4 and 5 - insoluble protein fractions 2 and 4 h after induction. The SDS - PAGE image D represents cellular protein fractions 4 h after batch 6 × His-MBP-RI protein production when induction was carried in batch cultures of MSM medium at OD600 = 1. Numbered SDS-Page gel lanes represent protein fractions 4 h after induction: 1, 4 and 7 - total; 2, 5 and 8 - soluble; 3, 6 and 9 - insoluble protein fractions. The gel lanes marked with M represent the protein size marker PageRuler™ Protein Ladder Plus from Fermentas Ltd.

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Microbial Cell Factories

ISSN: 1475-2859

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