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Figure 5 | Microbial Cell Factories

Figure 5

From: Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures

Figure 5

Shake flask fed-batch culture growth of E. coli RV308/p ibpfxsT7lucA /pCTUT7MBP-RI with and without target protein production. (A) Growth curves (OD600) of independent fed-batch cultivations at 37°C and the calculated corresponding specific growth rate μ (B). Induction of 6xHis-MBP-RI synthesis was performed at the selected time points when the specific growth rate was about 0.33 h-1, 0.22 h-1, or 0.1 h-1, respectively. The culture cultivation curves were generated from three independent cultivation experiments.

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Microbial Cell Factories

ISSN: 1475-2859

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