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Figure 4 | Microbial Cell Factories

Figure 4

From: Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures

Figure 4

SDS - PAGE images of total, soluble and insoluble cellular protein fractions normalized to biomass quantity of the 7 h samples of 96 microwell plate cultures of the RI expression library consisting of 45 different expression vectors propagated in E. coli RV309 p ibpfxsT7lucA by the EnBase® technology performed at 37°C. The gel lanes are numbered in respect to the expression system: 1 - pCT7, 2 - pClac, 3 - pCVar, 4 - pCUT7, 5 - pCUlac, 6 - pCUvar, 7-pCTUT7, 8-pCTUlac, 9-pCTUVar. Rows: RI produced in total soluble and insoluble protein fractions, tagged with the 6 × His tag (size of fusion protein 50 kDa) (A1-A3); 6 × His-GST (68 kDa) (B1-B3); 6 × His-MBP (81 kDa) (C1-C3); 6 × His-SUMO (64 kDa) (D1-D3); 6 × His-Trx (62 kDa) (E1-E3). Lanes marked with letter M show the protein size marker PageRuler™ Protein Ladder Plus from Fermentas Ltd.

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Microbial Cell Factories

ISSN: 1475-2859

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