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Figure 3 | Microbial Cell Factories

Figure 3

From: Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures

Figure 3

Aggregation signal measured as luminescence in samples of the RI expression library consisting of 45 different expression vectors propagated in E. coli RV309 p ibpfxsT7lucA in 96 microwell plates by the EnBase® technology 7 hours after induction. The lower graph (A) represents luminescence values, reflecting tagged RI protein misfolding stress levels of all expression systems 7 hours after induction with 0.5 mM IPTG. The luminescence values generated by overexpressed luciferase are derived from luciferase activity measurement from cultures performed at 37°C (full set of values displayed) fragments of results derived from cultures performed at 30 and 22°C, for which only the soluble fusion protein giving expression platforms are presented. The bars are numbered in respect to the expression system: 1 - pCT7, 2 - pClac, 3 - pCVar, 4 - pCUT7, 5 - pCUlac, 6 - pCUvar, 7-pCTUT7, 8-pCTUlac, 9-CTUVar. The upper graphs (B, C and D represent soluble fusion protein amounts in mg per gram of cell dry weight [mg (gCDW)-1], of the cultures with the 6 × His-MBP-RI fusion constructs at 37°C 30°C and 22°C which gave the lowest luminescence signal.

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