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Figure 2 | Microbial Cell Factories

Figure 2

From: Novel approach of high cell density recombinant bioprocess development: Optimisation and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures

Figure 2

Growth of the RI expression library consisting of 45 different expression vectors propagated in E. coli RV309 p ibpfxsT7lucA in 96 microwell plates by the EnBase® technology. (A) Inocculum preparation and (B) 96 microwell plate expression cultures with plates which were non-induced (closed circle) or set to different postinduction temperatures at the time of induction (37°C - red, 30°C - green, 22°C - blue). Bars indicate the SD of all cultures of a whole plate (including all different vectors). OD600 - circles, specific growth rate - squares (average specific growth rate in the time period before).

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