Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin

Table 2 Purification of insulin precursor from microbial cultures

Expression host	Chromatography matrix	Recovery (%)^a	Purity (%)^a	Reference^b
E. coli ^c	IgG-Sepharose	90	nd ^d	[10]
S. cerevisiae	Streamline SP (XL) Expanded bed adsorption	88	nd	[43]
P. pastoris	XAD-7→ Sephadex-G50	>80 ^e	60 ^e	[26]
P. pastoris	CM-Sepharose FF	97	97	[27]
P. pastoris	Streamline SP (XL) Expanded bed adsorption	55	97	Present study
P. pastoris	IMAC-coupled with copper ions	95	96	Present study

^a Recovery and purity relates to the first chromatographic step
^b Only references on insulin precursor purification included containing respective quantitative data
^c Insulin precursor fused to IgG binding domains produced in the form of inclusion bodies
^d nd; not determined
^e Recovery and purity relates to the two-step purification procedure

ISSN: 1475-2859