Figure 5From: A novel prokaryotic vector for identification and selection of recombinants: Direct use of the vector for expression studies in E. coliSDS-PAGE showing protein expression from one glowing and one non-glowing clones of p24MGFPm-MAP, p24MGFPm-GCSF and p24MGFPm-SAK in non-amber suppressor strain BL21 E. coli cells. M: Protein molecular marker, lanes 1, 3, 5: Expression of foreign genes from non-glowing colony containing MAP-GFP, GCSF-GFP and SAK-GFP constructs respectively. Lanes 2, 4, 6: SDS-PAGE profile of glowing colonies from each construct.Back to article page