Effect of introduction of stop codon upstream of GFP gene on the fluorescence. A. Clone map of the pBAD24MM where a stop codon was inserted between. NdeI and EcoRI site. B and C are the fluorescence of GFP upon induction expressed from pBAD24M-GFP and pBAD24MMGFP, respectively. Note the complete loss of GFP fluorescence with pBAD24MM-GFP.