Figure 7

In-gel detection of IFP fusion proteins expressed in L. tarentolae. A) Purified IFP-His fusion protein, BSA and purified GST were separated by SDS-PAGE. In-gel detection was performed directly in the cast gel (lower panel), followed by Coomassie staining for visualization of all protein bands (upper panel). Partial protein degradation was observed in the IR scan, most likely because the metal protease inhibitor EDTA was omitted (to preserve the HisTrap HP columns used). B) Abundance of ANAC42-IFP-His protein during affinity chromatography was tested by analyzing aliquots of the following fractions: C1, 2, 3, unbound protein after each circulation; W1, 3, protein in wash solution; E1 - E4, protein after elution. Proteins were separated by SDS-PAGE and scanned in-gel (lower panel) and subsequently subjected to Coomassie staining (upper panel). M, molecular size marker.