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Figure 5 | Microbial Cell Factories

Figure 5

From: A highly efficient pipeline for protein expression in Leishmania tarentolae using infrared fluorescence protein as marker

Figure 5

Stability and solubility analysis of IFP expressed in L. tarentolae. IFP-His expressing Leishmania cells were sonicated in Tris and PBS buffer containing a protease inhibitor cocktail supplemented with EDTA (+Prot. Inh. + EDTA), an EDTA-free protease inhibitor cocktail (+Prot. Inh. - EDTA) or no protease inhibitors (- Prot. Inh.), and ultracentrifuged. Crude extracts (C) before ultracentrifugation, as well as pellets (P) and soluble supernatants (S) after ultracentrifugation were IR-scanned in the wells of a 96-well microtiter plate. (-) 100 μL ANAC42-His expressing Leishmania cells, negative control for IR signal; (+) 100 μL IFP-His expressing cells, positive control for IR signal.

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Microbial Cell Factories

ISSN: 1475-2859

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